Orthogonal aaRS/tRNA Pair Identification
Identification of functionally independent tRNA/aaRS pairs that minimize cross-reactivity with host systems.
Modular Engineering
Platform
Customizable Service
Packages
In-House Library
Generation
Workflow
Integration
High-Fidelity
Quality Control
Protheragen is a leading provider of preclinical services and a pioneering biotech company focused on enabling site-specific incorporation of non-canonical amino acids (ncAAs) through high-throughput orthogonal tRNA/aaRS pair development, offering powerful tools for pharmaceutical R&D, synthetic biology, diagnostics, and more.
With a multidisciplinary team and a strong foundation in molecular biology, protein chemistry, and genome engineering, we offer scalable and modular solutions to support academic and industrial partners. Our goal is to advance genetic code expansion technologies and provide robust tools for the development of next-generation biomolecules.
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Identification of functionally independent tRNA/aaRS pairs that minimize cross-reactivity with host systems.
Evaluation of orthogonality and incorporation fidelity in cell-free systems to support downstream in vivo studies.
Implementation of ncAA incorporation strategies in E. coli, yeast, and mammalian systems to support functional studies and product development.
Deliver custom-engineered molecules via site-specific ncAA insertion powered by proprietary GCEngine™ platform for academic research and preclinical biotherapeutics.
Facilitates the precise insertion of structural and functional ncAAs to enhance proteolytic stability, enforce specific macrocyclization conformations, or introduce distinct bio-orthogonal handles for structural biology and drug discovery pipelines.
Enables site-specific modifications to dissect enzyme mechanisms, create tailored post-translational modification (PTM) mimics, or optimize biophysical properties and therapeutic efficacy without disrupting native tertiary structures.
Drives the creation of homogenous, site-specific antibody-drug conjugates (ADCs) and bispecific constructs through the high-fidelity insertion of click-chemistry handles, ensuring precise payload-to-antibody ratios (DAR) and optimized pharmacokinetics.
Tailored Solutions Powered by Deep tRNA Engineering
Standard, mutant, and unnatural tRNAs are available, supported by in-house oligo synthesis and gene optimization services.
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We offer both natural and unnatural chemical modifications, along with site-specific editing for functional enhancement.
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Includes integrity assessment, purity evaluation, concentration analysis, and gDNA contamination detection/removal.
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Our purification options include gel-based, affinity-tag, and native purification for both prokaryotic and eukaryotic tRNAs.
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Pharmaceutical R&D
Enables the development of advanced therapeutic peptides, proteins, and antibodies by integrating non-canonical amino acids to improve efficacy, specificity, and pharmacological properties across diverse disease areas.
Synthetic Biology
Expands the chemical and functional diversity of biological systems through site-specific ncAA incorporation, supporting the design of novel biomolecules with enhanced or unnatural functionalities.
Diagnostics & Biosensing
Facilitates the creation of sensitive and customizable biosensors using ncAA-modified peptides and proteins for precise detection and high-throughput analytical applications.
A specialized platform advancing genetic code expansion through orthogonal tRNA/aaRS technologies, enabling precise ncAA incorporation for biotherapeutic development, synthetic biology, and diagnostics.
